Figure 4

Artemis transcription plots of characteristic p44 transcription profiles (Red: Ap-HL-60, Green: Ap-HMEC-1, Blue: Ap-ISE6; plots were "smoothed" by setting the sliding window average to 5). Arrows in panel B indicate p44 conserved sequence "horns" on the coding (minus) strand, and "reflected" horns (panel A) in the anti-sense (plus) strand. A strong signal (green) associated with the HVR in APH_1342 (*), likely indicates expression of the corresponding p44 paralog (p44-31) in HMEC-1. The lack of HVR associated signal in APH_1343, but strong conserved sequence associated signals (horns), is typical of most p44 paralogs. An unannotated segment of p44 conserved sequence lies between APH_1343 and APH_1344 (yellow) on the minus strand. It also showed strong sense (B) and anti-sense (A) signals. APH_1344 and APH_1345 show typical transcription profiles: signal on the sense strand (B) but not on the anti-sense strand (A).