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Table 1 Confirmation of previously identified Zap1 target genes by E1, E2, and E3 clustering.

From: Differential control of Zap1-regulated genes in response to zinc deficiency in Saccharomyces cerevisiae

ORFa Gene Function Fold induction ZRE
    E1b E2b E3-1c E3-2c startd scoree sequence
Experimentally tested Zap1 target genes
YDR284C DPP1 diacylglycerol pyrophosphate phosphatase 5.5 3.6 2.4 2.2 -452 9.6 ACCTGAAAGGT
YDR492W IZH1 membrane steroid hormone receptor ortholog 2.8 4.4 1.4 1.6 -914 5.0 TCCCTCAATGA
        -417 11.2 ACCCTAAAGGT
YGL255W ZRT1 plasma membrane zinc uptake transporter 24.2 18.9 38.8 34.4 -456 9.3 ACCTTTGGGGT
        -338 7.7 ACCTCGAAGGA
        -319 12.0 ACCTTGAGGGT
        -204 11.7 ACCTTGAAGGT
YGL256W ADH4 alcohol dehydrogenase IV 26.2 5.6 12.6 12.6 -269 9.4 ACCGTGAAGGT
        -191 5.3 GCCTTCAATGA
YJL056C ZAP1 zinc-responsive transcriptional activator protein 7.5 16.9 96.5 79.7 -144 11.2 ACCCTAAAGGT
YKL175W ZRT3 vacuolar zinc export transporter 7.9 8.7 4.9 4.8 -155 11.6 ACCTTAAGGGT
YLR130C ZRT2 plasma membrane zinc uptake transporter 5.6 12.3 2.7 2.5 -941 6.1 ACCCTAACTGT
        -311 11.2 ACCCTAAAGGT
        -262 11.2 ACCCTAAAGGT
        -174 5.8 ACCTTTTGGGA
        -112 4.9 ACCAACAGGGT
        -41 5.3 GCCTGCAATGT
YMR243C ZRC1 vacuolar zinc import transporter 2.1 4.7 1.6 1.8 -174 9.6 GCCTTGAAGGT
YMR319C FET4 plasma membrane Fe/Cu/Zn uptake transporter 2.3 2.4 1.6 1.7 -384 7.8 ACCCCACGGGT
YNR039C ZRG17 endoplasmic reticulum zinc import transporter 3.2 3.5 2.3 1.9 -527 11.7 ACCTTGAAGGT
YOL002C IZH2 membrane steroid hormone receptor ortholog 1.9 2.0 3.4 3.3 -256 7.7 ACCCTAGAGGA
        -173 7.2 ACCCCGAGTGT
YOL154W ZPS1 cell wall protein, putative metalloprotease 13.9 10.1 78.6 57.9 -329 11.8 ACCTTCAGGGT
        -314 11.8 ACCTTCAGGGT
Likely Zap1 target genes
YBL049W MOH1 zinc-binding protein, function unknown 10.7 2.2 3.2 3.8 -387 8.7 CCCTTGAGGGA
YBR117C* TKL2* transketolase, pentose phosphate pathway 5.4 2.0 1.9 2.1 -844 8.2 ACCTTATGGGT
        -524 5.3 ACCCAAAATGT
YDR055W* PST1* cell wall protein 5.0 2.7 2.0 1.9 -886 7.4 ACCCCAAGGGA
        -405 8.0 TCCTTGAGGGA
YGL121C GPG1 putative gamma subunit of a heterotrimeric G protein 10.8 2.7 2.1 2.4 -287 8.3 CCCTTCGAGGT
        -208 9.0 ACCGTAAAGGT
YGL257C MNT2 mannosyltransferase, O-linked glycosylation 2.3 4.4 4.0 3.7 -572 5.3 GCCTTCAATGA
        -494 9.4 ACCGTGAAGGT
YGL258W VEL1 function unknown 16.3 4.0 112.6 78.6 -763 6.3 ACCTTGCATGA
        -232 10.4 ACCCTGCGGGT
YGR295C COS6 function unknown 2.7 2.2 1.5 1.6 -875 5.0 AACTTAAATGT
        -309 9.1 ACCTTAAATGT
YJL132W*   similar to phospholipase D 5.2 3.8 3.3 2.8 -154 7.8 ACCCAAAGGGT
YJR061W   Mnn4-related, N-linked glycosylation 4.6 2.1 2.0 1.8 -278 9.8 ACCTTCAAGGA
YKL165C MCD4 glycosylphosphatidylinositol (GPI) anchor synthesis 4.1 2.5 1.8 1.8 -104 11.4 ACCTTAAAGGT
YLL020C   function unknown 5.1 3.1 1.7 1.6 -933 12.0 ACCTTGAGGGT
YMR120C ADE17 AICAR transformylase, purine biosynthesis 5.2 3.7 1.9 2.2 -98 8.7 ACCTTTAGTGT
YMR271C URA10 orotate phosphoribosyltransferase, pyrimidine biosynthesis 6.1 2.5 4.9 5.2 -143 7.9 ACCTTTCGGGA
YMR297W PRC1 vacuolar carboxypeptidase Y 3.0 3.5 1.5 1.9 -182 8.1 ACCCCGCGGGT
YNL254C   function unknown 9.7 12.0 2.3 2.0 -432 11.7 ACCTTGAAGGT
YNL336W COS1 function unknown 2.1 2.0 1.6 1.6 -871 5.0 AACTTAAATGT
        -591 5.3 AACCTAGAGGT
        -314 9.1 ACCTTAAATGT
YOL084W PHM7 major facilitator superfamily member, function unknown 9.7 2.1 2.5 2.3 -766 11.7 ACCTTGAAGGT
YOL131W   function unknown 5.0 2.3 2.5 2.2 -1000 7.6 AACTTCAGGGT
        -458 8.2 ACCTGGAAGGA
        -364 5.9 ACCCAAGAGGT
        -122 5.5 TCCATTAGGGT
YOR387C   function unknown 17.8 19.3 136.4 113.6 -763 6.3 ACCTTGCATGA
        -231 10.4 ACCCTGCGGGT
YOR134W BAG7 GTPase-activating protein, control of cell wall synthesis 5.9 2.0 1.7 1.6 -445 6.0 CCCTCCAAGGA
        -325 8.6 CCCCTGCAGGT
YPL250C* ICY2* function unknown 2.1 2.3 3.1 3.1 -756 8.9 CCCTTCCGGGT
        -524 7.4 ACCCCAAGGGA
        -475 5.7 GCCCAAAGGGT
        -360 5.6 CCCGTCAGTGT
  1. a) Results for genes marked with asterisks were confirmed independently by S1 nuclease protection assay in Figure 2.
  2. b) Expression ratios are the average of two independent microarray experiments (Lyons et. al. 2000).
  3. c) Results from two independent microarray experiments (E3-1 and E3-2) are shown.
  4. d) Numbers indicate the distance from the ATG initiation codon.
  5. e) Score calculated for each sequence with a position-specific scoring matrix generated by RSAT.