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Table 3 New candidate Zap1 target genes identified by E3 and E4 clustering.

From: Differential control of Zap1-regulated genes in response to zinc deficiency in Saccharomyces cerevisiae

ORFa Gene Function Fold inductionb ZRE
    E3-1 E3-2 E4-1 E4-2 startc scored sequencee
YDL125C* HNT1* adenosine 5'-monophosphoramidase 1.9 1.6 3.6 4.1 -279 6.9 GCCTCAAAGGT
YEL060C* PRB1* vacuolar proteinase B 2.6 2.8 2.0 2.1 -677 6.5 GCCATGAGGGT
YFL014W HSP12 cell wall protein, stress resistance 1.5 1.5 4.4 3.7 -211 5.0 ACCTCAAAGTT
YGR254W ENO1 enolase I, glycolysis and gluconeogenesis 2.6 2.9 1.4 1.7 -927 9.4 ACCGTGAAGGT
        -371 5.1 ACCTGAGCGGT
        -216 5.6 CACCTCAAGGT
YGR279C* SCW4* cell wall protein with similarity to glucanases 1.8 1.9 1.5 1.4 -481 5.4 CCCTGCACGGT
        -466 6.3 ACCCTCTGGGA
YHR174W* ENO2* enolase II, glycolysis and gluconeogenesis 2.5 2.8 1.3 1.6 -594 4.8 ACGCTGCGGGT
YIL169C*   cell wall potein 3.4 3.1 2.1 2.2 -742 10.8 ACCCGGAAGGT
        -252 6.0 ACCTCGCAGGC
YJL052W TDH1 glyceraldehyde-3-phosphate dehydrogenase 1.4 1.6 1.6 1.6 -347 4.6 ACCTTCGGAGT
YJL171C   cell wall protein 1.6 1.8 1.6 1.8 -573 4.9 CCCATAAAGGA
YKR042W* UTH1* mitochondrial protein, oxidative stress resistance 3.5 4.0 1.4 1.5 -282 6.9 CCCTTCAATGT
YLL053C   aquaporin 1.6 1.5 1.7 1.5 -354 5.5 ACCGGTCGGGT
YMR169C ALD3 aldehyde dehydrogenase 2.0 1.9 1.6 1.5 -214 9.1 TCCCTAAGGGT
        -79 4.6 ACCTGGCATGA
YOR348C PUT4 proline permease 1.8 1.8 1.9 1.8 -299 8.2 CCCTGCAAGGT
YPL274W* SAM3* S-adenosylmethionine permease 1.9 1.7 2.1 2.2 -240 4.7 TCCCCTGCGGT
YPR003C   putative ER sulfate permease 2.2 2.1 1.5 1.5 -149 5.5 ACCGAAAAGGT
  1. a) Results for genes marked with asterisks were confirmed independently by S1 nuclease protection assay in Figure 2.
  2. b) Results from two independent microarray experiments (E3-1, E3-2; E4-1, E4-2) for each condition are shown.
  3. c) Numbers indicate the distance from the ATG initiation codon.
  4. d) Score calculated for each sequence with a position-specific scoring matrix generated by RSAT.
  5. e) ZREs shown in bold were tested for interaction with Zap1 as described in Figure 3A.