Figure 4

EvoPrinter and cis -Decoder analysis of the Drosophila nervy neural precursor cell enhancer region. EvoPrinter (A) and cis-Decoder (B) analysis of a nervy neural precursor cell enhancer positioned 90 bp upstream from the nervy transcriptional start site. A) Shown is an EvoPrint of the Drosophila melanogaster nervy gene 5' flanking DNA (487 bp). The predicted nervy transcriptional start site is denoted with an arrow. Test species included in the comparative analysis were D. simulans, D. sechellia, D. erecta, D. yakuba, D. ananassae, D. persimilis, D. pseudoobscura, D. willistoni and D. grimshawi. Black capital letters represent bases conserved in all, or all but one, species. Putative TFDNA-binding sites within the conserved sequences are highlighted (bHLH E-box sites, yellow; an Pbx/Extradenticle site, blue; and an Antennapedia class homeodomain binding site, green). B) Conserved sequence blocks identified in the nervy EvoPrint (A) were extracted and scanned for the presence of neural precursor cell enhancer c DTs. c DTs were generated using NB and PNS enhancer CSBs listed in Table 1. c DTs generated by the inclusion of the nervy CSBs in the c DT library construction are also shown. CNS neuroblast specific cDTs are highlighted in red typeface, PNS precursor cell specific are noted with blue typeface and those present in both are indicated with black typeface (the number of enhancers that contain a c DT is also indicated).