Novel functional view of the crocidolite asbestos-treated A549 human lung epithelial transcriptome reveals an intricate network of pathways with opposing functions

Table 2 Validation of the microarray data from crocidolite-treated human A549 cells

Gene	Alias for Protein Product	Affymetrix* (fold change) this study	QRT-PCR (fold change) this study	Changes in mRNA levels in human cells observed by other groups
EGR1	Egr1	115.7	281.7 ± 3.8
ATF3	ATF3	25.3	23.7 ± 0.1
c-Jun	c-Jun	8.6	10.5 ± 0.7
JunB	JunB	2.2	3.6 ± 0.2
IL8	Interleukin 8	16.6	N.D.	Increased [73]
TP53	p53	1.8	N.D.	Increased [16, 74]
SOD2	Mn-SOD	2.2	N.D.	Increased [75]^‡
HMOX1	Heme oxygenase 1	2.1	N.D.	Increased [75, 76]^†
c-myc	myc	2.0	N.D.	Increased [77]
CDKN1A	p21, Cip1	1.9	N.D.	Increased [16, 74]

*represents significantly greater than control, q less than 0.05; N.D., not determined
^† chrysotile-treated A549 human lung epithelial cells
^‡ crocidolite-treated human pleural mesothelial cells

ISSN: 1471-2164