Characterization of hemizygous deletions in Citrus using array-Comparative Genomic Hybridization and microsynteny comparisons with the poplar genome

Table 1 Gene dosage measurement of deleted genes in 39B3 and 39E7 Citrus mutants.

Unigene	EST accession number (GenBank)	Array-CGH		Real-time PCR gene dosage
		Mutant/wt ratio	P value	Template 39B3	Template 39E7
aCL4690Contig1	CX295702	0.59	0.10	0.56 ± 0.02	0.99 ± 0.09
aCL1915Contig2	DY300024	0.62	0.12	0.60 ± 0.08	0.96 ± 0.04
aCL3317Contig1	DY265056	0.62	0.10	0.60 ± 0.03	0.98 ± 0.03
aC20009H03SK_c	CX308429	0.63	0.10	0.50 ± 0.02	0.98 ± 0.11
aCL766Contig1	CX288964	0.65	0.13	0.56 ± 0.06	0.96 ± 0.11
aCL7097Contig1	FC868864	0.65	0.12	0.59 ± 0.04	1.15 ± 0.07
aCL2087Contig2	DY300006	0.57	0.05	1.05 ± 0.13	0.59 ± 0.11
aCL6684Contig1	DY265447	0.60	0.05	1.04 ± 0.12	0.59 ± 0.12
aCL6641Contig1	FC930062	0.62	0.05	1.14 ± 0.19	0.58 ± 0.08
aCL3902Contig1	DY267778	0.62	0.05	1.12 ± 0.10	0.64 ± 0.04
aC05139C12SK_c	CX296347	0.66	0.05	1.24 ± 0.22	0.61 ± 0.05
aC01019E12SK_c	CX288357	0.66	0.05	1.04 ± 0.07	0.63 ± 0.11

Accession numbers and the corresponding assembled unigenes for several candidate ESTs are shown. Array-CGH data are signal ratios of mutant with respect to wild type samples for each single EST. Gene dosage was calculated through real-time PCR on 39B3 and 39E7 genomic templates. A horizontal line separates array-CGH hits of 39B3 mutant (upper) from those of the 39E7 mutant (lower).

ISSN: 1471-2164