Characterization of expressed sequence tags from a full-length enriched cDNA library of Cryptomeria japonica male strobili

Table 1 Summary of characteristics of the full-length cDNA library from male strobili of C. japonica

Total sequences	36,011
Number of 5'sequences	18,843
Number of 3' sequences	17,168
Number of cDNA clones	19,437
Number of contigs^a	7,686
Number of singlets^a	15,972
Number of unique transcripts^b	10,463
Number of unique transcripts corresponded to
1 clone	6,320
2 clones	2,078
3–5 clones	1,686
6–10 clones	353
11–20 clones	21
>21 clones	5

^a ESTs were assembled using the PHRAP program.
^b Contigs and singlets were grouped using the BLASTN program and then both 5'- and 3'- end sequences derived from the respective same clones were grouped together.

ISSN: 1471-2164