Maximum likelihood phylogenetic trees of ESAG5 and ESAG2 show that ESAG5 sequences from T. b. gambiense 972 (Tbg) cluster separately from those from T. b. brucei EATRO 2340 (Tbb) and T. equiperdum STIB 818 (Teq). Most of the T. b. brucei ESAG2 sequences also appear to cluster separately. The ESAG DNA sequence types are as listed in Tables 1-3. Sequence accession numbers are listed in the Materials and Methods. Bootstrap support values are shown for key nodes only. Type A and Type B sequences referred to in the text are indicated with brackets.