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Table 2 T. brucei brucei EATRO 2340 BES TAR clone library.

From: Isolation and analysis of the genetic diversity of repertoires of VSG expression site containing telomeres from Trypanosoma brucei gambiense, T. b. brucei and T. equiperdum

BES Prom. type ESAG6 type No. TAR clones Represent. TAR clone ESAG5 type ESAG2 type
   DNA Protein    DNA Protein DNA Protein
1 1 1 1 11 F44 1 1 1 1
2 2 2 2 9 E42 2 2 2 2
3 3 3 3 9 G37 3 3 3 3
4 4 4a 4a 3 E16 4 4 -b -
5 5 5 5 19 G39 5c - -b -
6 5 6 6 5 K13 -d - -b -
7 6 7 7 1 V6 6 5 4 4
8 7 8 8 8 E51 7 6 5 5
9 8 9 9 7 S32 8 7 6e 6e
10 9 10 10 4 U19 9 8 7 7
11 9 11 and 12f 11 and 12f 1 S43 10 9 8 8
12 10 13 13 16 E23 11 10 9 9
13 11 14 14 8 J12 12 11 10 10
14 11 15 15 9 F36 13 12 11 11
15 12 16 16 17 E12 14 13 12 12
16 13 17 17 2 R23 10 9 8 8
17 13 11 11 2 E40 10 9 8 8
18 13 18 18 4 J4 10 9 13e 13e
19 14 19 19 13 E19 15 14 14 14
20 15 20 20 12 F35 16 15 15 15
21 16 21 21 16 E48 17 16 16 16
22 17 22 22 31 F27 18 17 17 17
23 18 11 11 1 E44 -b - -b -
Total 18 22 22 208   18 17 17 17
  1. Overview of T. b. brucei EATRO 2340 TAR clone library. A total of 208 TAR clones were isolated and typed into 23 different BES sets. The sequence typing proceeded further as described for Table 1. As before, two TAR clones were chosen for each BES type (the name of one clone is indicated in the chart), and the full length ESAG6, ESAG5 and ESAG2 ORFs were isolated and sequenced from both of these two clones.
  2. aFirst 1000 bp only.
  3. bPresence not detected by PCR. See Materials and Methods for diagnostic primers that were used for each ESAG.
  4. cPseudogene.
  5. dPresent but not sequenced.
  6. eOne clone.
  7. fTwo ESAG6 in one BES, both assembled.