Isolation and analysis of the genetic diversity of repertoires of VSG expression site containing telomeres from Trypanosoma brucei gambiense, T. b. brucei and T. equiperdum

Table 2 T. brucei brucei EATRO 2340 BES TAR clone library.

BES	Prom. type	ESAG6 type		No. TAR clones	Represent. TAR clone	ESAG5 type		ESAG2 type
		DNA	Protein			DNA	Protein	DNA	Protein
1	1	1	1	11	F44	1	1	1	1
2	2	2	2	9	E42	2	2	2	2
3	3	3	3	9	G37	3	3	3	3
4	4	4^a	4^a	3	E16	4	4	-^b	-
5	5	5	5	19	G39	5^c	-	-^b	-
6	5	6	6	5	K13	-^d	-	-^b	-
7	6	7	7	1	V6	6	5	4	4
8	7	8	8	8	E51	7	6	5	5
9	8	9	9	7	S32	8	7	6^e	6^e
10	9	10	10	4	U19	9	8	7	7
11	9	11 and 12^f	11 and 12^f	1	S43	10	9	8	8
12	10	13	13	16	E23	11	10	9	9
13	11	14	14	8	J12	12	11	10	10
14	11	15	15	9	F36	13	12	11	11
15	12	16	16	17	E12	14	13	12	12
16	13	17	17	2	R23	10	9	8	8
17	13	11	11	2	E40	10	9	8	8
18	13	18	18	4	J4	10	9	13^e	13^e
19	14	19	19	13	E19	15	14	14	14
20	15	20	20	12	F35	16	15	15	15
21	16	21	21	16	E48	17	16	16	16
22	17	22	22	31	F27	18	17	17	17
23	18	11	11	1	E44	-^b	-	-^b	-
Total	18	22	22	208		18	17	17	17

Overview of T. b. brucei EATRO 2340 TAR clone library. A total of 208 TAR clones were isolated and typed into 23 different BES sets. The sequence typing proceeded further as described for Table 1. As before, two TAR clones were chosen for each BES type (the name of one clone is indicated in the chart), and the full length ESAG6, ESAG5 and ESAG2 ORFs were isolated and sequenced from both of these two clones.
^aFirst 1000 bp only.
^bPresence not detected by PCR. See Materials and Methods for diagnostic primers that were used for each ESAG.
^cPseudogene.
^dPresent but not sequenced.
^eOne clone.
^fTwo ESAG6 in one BES, both assembled.

ISSN: 1471-2164