Isolation and analysis of the genetic diversity of repertoires of VSG expression site containing telomeres from Trypanosoma brucei gambiense, T. b. brucei and T. equiperdum

Table 5 BES amino acid diversity.

Locus	Strain	N° seqs	Codons	Polymorphic Sites % S	Average a.a. diversity π	dN/dS ratio ω > 2	Average dN/dS ω (avg)
ESAG6	T. b. g.	12	401	9.2	0.018	15	3.40
	T. b. b.	23		24.7	0.079	24	3.20
	T. eq.	8		9.2	0.040	38	2.15
ESAG5	T. b. g.	13	469	2.8	0.005	0	n.a.
	T. b. b.	19		27.5	0.087	48	3.75
	T. eq.	10		19.4	0.087	49	2.29
ESAG2	T. b. g.	11	424	19.1	0.059	15	3.38
	T. b. b.	19		37.0	0.130	11	3.98
	T. eq.	10		19.6	0.053	11	4.39

Amino acid diversity and selection in ESAG sequences from three trypanosomatid protozoa. The BES loci studied are indicated on the left. The strains are Trypanosoma brucei gambiense DAL 972 (T.b.g.), T. b. brucei EATRO 2340 (T.b.r.) and T. equiperdum STIB 818 (T. eq.). N° seqs. = number of protein sequences in alignment; codons = number of codons in gap-stripped alignment; % S = percentage of polymorphic sites; π = average pairwise amino acid diversity; dN/dS ratio ω > 2 = number of amino acid residues with dN/dS ratio (ω) estimated to be greater than 2 indicating evidence for positive selection; Average dN/dS ω (avg.) = average ω estimate over residues with ω > 2.

ISSN: 1471-2164