Dynamic RNA profiling in Plasmodium falciparum synchronized blood stages exposed to lethal doses of artesunate

BMC Genomics

Table 2 Differentially expressed chaperone and chaperone-related genes in artesunate-treated cells

Gene ID	Description	Pexel	family	ref	ART 90 mn	ART 3 h	HS
PFE1605w	DNAJ protein	yes	resa like/dnaJ	1, 3		1,01
PFD0080c	hypothetical protein, conserved in P.falciparum	yes	resa like	3	1,31	1,82	+
PF10_0378	DnaJ protein, putative	yes	Hsp40, type III	1, 2, 4	1,33	1,53	+
PFE0040c	mature parasite-infected erythrocyte surface antigen (MESA) or PfEMP2	yes	Hsp40, type IV	2, 4	0,92	1,87
PFA0660w	protein with DNAJ domain, dnj1/sis1 family	yes	Hsp40, type II	1, 2, 4	1,01	1,34	+
MAL7P1.7	RESA-like protein	yes	resa like	3		0,83
PFL0050c	hypothetical protein	yes	resa like	3		0,86
PF07_0029	heat shock protein 86	no	hsp90	1		0,9	+
PF07_0030	heat shock protein 86 family protein	no	Hsp86 family	5		1,63	+
PF08_0054	heat shock 70 kDa protein	no	hsp70	1		0,88	+
PF11_0034	hypothetical protein	yes	Hsp40, type IV	1, 2, 4		0,81	+
PFA0675w	P. falciparum RESA-like protein with DnaJ domain	yes	Hsp40, type IV	1, 2, 4		1,96	+
PF14_0700	hypothetical protein, conserved	no	Hsp40, type III	1, 2, 4		0,81
PF11_0509	ring-infected erythrocyte surface antigen putative	yes	Hsp40, type IV	1, 2, 4		-1,14
PF13_0102	DNAJ-like Sec63 homologue	no	Hsp40, type III	1, 2, 4		-1,04
PF11_0216	hypothetical protein	no	Heat shock factor binding protein 1	6		-0,93	-
PFC0975c	PFCYP19, cyclophilin, peptidyl-prolyl cis-trans isomerase	no	cyclophillin	1		-0,97

Gene ID: identifier as found in PlasmoDB.
Art columns: log ratios of gene expression under artesunate exposure at 90 minutes and 3 hours.
HS column: over (+) or under (-) expression.
Results were compared with transcriptome modifications induced by heat-shock (HS) [12] and chloroquine (CQ) [8].
Chaperone- and chaperone-related genes were defined based on: 1) Acharya et al. [19], 2) Botha et al. [20], 3) resa-like annotation from GeneDB, 4) genes fitting the HMM profile of DNAJ (PF00226) from Pfam, 5) The Hsp86 family annotation from GeneDB, 6) genes fitting the HMM profile of heat shock factor binding protein 1 (PF06825) from Pfam.

ISSN: 1471-2164