Dynamic RNA profiling in Plasmodium falciparum synchronized blood stages exposed to lethal doses of artesunate

BMC Genomics

Table 9 Differentially expressed genes encoding proteins with Zn-finger motifs in artesunate-treated cells

Gene ID	Description	Zn-finger	ART 90 min	ART 3 h	HS	CQ
PFE1245w	hypothetical protein, conserved	Zinc finger C-x8-C-x5-C-x3-H type (PF00642)	1,28	0,91	+	+
PF14_0236	hypothetical protein	Zinc finger C-x8-C-x5-C-x3-H type (PF00642)		0,89	+
PFC0680w	hypothetical protein, conserved	Zinc finger C-x8-C-x5-C-x3-H type (PF00642)		1,55	+
PF10_0186	hypothetical protein	Zinc finger C-x8-C-x5-C-x3-H type (PF00642)		0,98
MAL13P1.122	hypothetical protein, conserved	PHD-finger (IPR001965)		0,81
PF14_0197	hypothetical protein	DNL zinc finger (PF05180)		0,87
PFI0470w	FHA domain protein, putative	C3HC4 type (RING finger) (PF00097)	0,89	1,05
PF10_0046	hypothetical protein	C3HC4 type (RING finger) (PF00097)		1,17
PFF0165c	hypothetical protein, conserved	C3HC4 type (RING finger) (PF00097)		0,94
PFL0440c	hypothetical protein, conserved	C3HC4 type (RING finger) (PF00097)		0,95
PFB0140w	hypothetical protein	DHHC zinc finger domain (PF01529)		-0,91
MAL13P1.76	TFIIH basal transcription factor subunit	TFIIH C1-like domain (PF07975)		-0,83

Gene ID: identifier as found in PlasmoDB.
Art columns: log ratios of gene expression under artesunate exposure at 90 minutes and 3 hours.
HS and CQ columns: over (+) expression.
Results were compared with transcriptome modifications induced by heat-shock (HS) [12] and chloroquine (CQ) [8].
Genes containing Zn-motifs were defined as genes fitting the following Zn-finger HMM profiles: B-box zinc finger (PF00643), C3HC zinc finger-like (PF07967), C3HC4 type (RING finger) (PF00097), CHY zinc finger (PF05495), CSL zinc finger (PF05207), CW-type Zinc Finger (PF07496), DHHC zinc finger domain (PF01529), DNL zinc finger (PF05180), FYVE zinc finger (PF01363), HIT zinc finger (PF04438), MIZ/SP-RING zinc finger (PF02891), MYND finger (PF01753), PHD-finger (PF00628), Putative zinc finger motif, C2HC5-type (PF06221), Sec23/Sec24 zinc finger (PF04810), SWIM zinc finger (PF04434), TFIIH C1-like domain (PF07975), Tim10/DDP family zinc finger (PF02953), U1 zinc finger (PF06220), Zinc finger C-x8-C-x5-C-x3-H type (and similar)(PF00642), Zinc finger found in FPG and IleRS (PF06827), Zinc finger, C2H2 type (PF00096), Zinc finger, ZZ type (PF00569), ZN-finger, Zn-finger in Ran binding protein and others (PF00641), Zn-finger in ubiquitin-hydrolases and other protein (PF02148), ZPR1 zinc-finger domain (PF03367), AN1-like Zinc finger (PF01428), C2H2 and C2HC zinc fingers (SSF57667), PHD-finger (IPR001965).

ISSN: 1471-2164