Marker | Forward oligo (5' to 3') | Reverse oligo (5' to 3') | Cycle conditions |
---|---|---|---|
Hox1 | GCATTGGGCCTTAATGAAACCC | CTTCTGCTTCATACGTCGAT | 95°C (3'). [94 (30"). 56°C. (30"). 72°C(1')]x34. 72°C. (3') |
Hox2 | CGGACTGCTTACACCAACACC | TCGGCGCTTGTTACGTCACA | 95°C (3'). [94 (30"). 55°C. (30"). 72°C(1')]x30. 72°C. (3') |
Hox4 | ACGCGACACCAGGTACTTGAA | ATATGCACGGCCGTGGGAAA | 95°C (3'). [94 (30"). 57°C. (30"). 72°C(1')]x30. 72°C. (3') |
Hox10 | GCAAGAAACGAGTGCCGTACA | CTTCACTTGACGGTCGGTAAG | 95°C (3'). [94 (30"). 57°C. (30"). 72°C(1')]x30. 72°C. (3') |
Wd-40 | TAGCTCGAGTTTGGGATATG | TGGGTTAAGAGGGTGAGTGG | 95°C (5'). [94 (1'). 54°C. (2'). 72°C(3')]x35. 72°C. (10'). 72°C. (3') |
0100134706A | TGTTCAGACCAGCATTACTGGC | GAGATCGCATTACGGACATTG | 95°C (3'). [94 (30"). 53°C. (30"). 72°C(1')]x30. 72°C. (3') |
EvxA | GGCCAACGTGCGTCGTTAT | ACGGCCACGTCTGCCGTTGT | 95°C (3'). [94 (30"). 55°C. (30"). 72°C(1')]x30. 72°C. (3') |