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Table 4 Markers, primers and thermal cycle conditions.

From: SNPs and Hox gene mapping in Ciona intestinalis

Marker

Forward oligo (5' to 3')

Reverse oligo (5' to 3')

Cycle conditions

Hox1

GCATTGGGCCTTAATGAAACCC

CTTCTGCTTCATACGTCGAT

95°C (3'). [94 (30"). 56°C. (30"). 72°C(1')]x34. 72°C. (3')

Hox2

CGGACTGCTTACACCAACACC

TCGGCGCTTGTTACGTCACA

95°C (3'). [94 (30"). 55°C. (30"). 72°C(1')]x30. 72°C. (3')

Hox4

ACGCGACACCAGGTACTTGAA

ATATGCACGGCCGTGGGAAA

95°C (3'). [94 (30"). 57°C. (30"). 72°C(1')]x30. 72°C. (3')

Hox10

GCAAGAAACGAGTGCCGTACA

CTTCACTTGACGGTCGGTAAG

95°C (3'). [94 (30"). 57°C. (30"). 72°C(1')]x30. 72°C. (3')

Wd-40

TAGCTCGAGTTTGGGATATG

TGGGTTAAGAGGGTGAGTGG

95°C (5'). [94 (1'). 54°C. (2'). 72°C(3')]x35. 72°C. (10'). 72°C. (3')

0100134706A

TGTTCAGACCAGCATTACTGGC

GAGATCGCATTACGGACATTG

95°C (3'). [94 (30"). 53°C. (30"). 72°C(1')]x30. 72°C. (3')

EvxA

GGCCAACGTGCGTCGTTAT

ACGGCCACGTCTGCCGTTGT

95°C (3'). [94 (30"). 55°C. (30"). 72°C(1')]x30. 72°C. (3')