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Table 4 The TaqMan-based DNA test for the K allele validated on late feathering K/K and K/k+ animals.

From: Partial duplication of the PRLR and SPEF2 genes at the late feathering locus in chicken

Animal ID Known
Genotype
Experiment 1
ΔCt
Experiment 1
Genotype
Experiment 2
ΔCt
Experiment 2
Genotype
 
2864 K/k+ 0.76 K/k+ 0.64 K/k+  
B2L4 K/k+ 0.68 K/k+ 0.49 Unassigned  
942 K/k+ 0.90 K/k+ 1.01 K/k+  
2855 K/k+ 0.98 K/k+ 0.87 K/k+  
4117 K/k+ 1.10 K/k+ 0.40 Unassigned  
4118 K/k+ 0.98 K/k+ 0.83 K/k+  
4332 K/k+ 1.31 K/k+ 1.14 K/k+  
6388 K/k+ 1.06 K/k+ 0.77 K/k+  
6324 K/k+ 1.12 K/k+ 0.91 K/k+  
6130 K/K 2.44 K/K 1.84 K/K  
6297 K/K 2.09 K/K 1.40 Unassigned  
952 K/K 2.09 K/K 1.74 K/K  
1030 K/K 1.83 K/K 1.90 K/K  
2849 K/K 2.26 K/K 1.64 K/K  
6187 K/K 2.10 K/K 1.85 K/K  
6242 K/K 1.73 unassigned 1.50 K/K  
6172 K/K 1.93 K/K 1.47 Unassigned  
  Experiment 1 Experiment 2 Total
  Animals (n = 17) % Animals (n = 17) % Animals (n = 34) %
Correct 16 94.1 13 76.5 29 85.3
Incorrect 0 0.0 0 0.0 0 0.0
Unassigned 1 5.9 4 23.5 5 14.7
  1. The seventeen animals were validated based on the ranges found for K/K and K/k+. For experiment 1, the ΔCt range for K/K was 0.68–1.43 and for K/k+ 1.75–2.50. For experiment 2, the ΔCt range for K/K was 0.63–1.24 and for K/k+ 1.50–2.10.
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