Partial duplication of the PRLR and SPEF2 genes at the late feathering locus in chicken

BMC Genomics

Table 4 The TaqMan-based DNA test for the K allele validated on late feathering K/K and K/k+ animals.

Animal ID	Known Genotype	Experiment 1 ΔCt	Experiment 1 Genotype	Experiment 2 ΔCt	Experiment 2 Genotype
2864	K/k+	0.76	K/k+	0.64	K/k+
B2L4	K/k+	0.68	K/k+	0.49	Unassigned
942	K/k+	0.90	K/k+	1.01	K/k+
2855	K/k+	0.98	K/k+	0.87	K/k+
4117	K/k+	1.10	K/k+	0.40	Unassigned
4118	K/k+	0.98	K/k+	0.83	K/k+
4332	K/k+	1.31	K/k+	1.14	K/k+
6388	K/k+	1.06	K/k+	0.77	K/k+
6324	K/k+	1.12	K/k+	0.91	K/k+
6130	K/K	2.44	K/K	1.84	K/K
6297	K/K	2.09	K/K	1.40	Unassigned
952	K/K	2.09	K/K	1.74	K/K
1030	K/K	1.83	K/K	1.90	K/K
2849	K/K	2.26	K/K	1.64	K/K
6187	K/K	2.10	K/K	1.85	K/K
6242	K/K	1.73	unassigned	1.50	K/K
6172	K/K	1.93	K/K	1.47	Unassigned
	Experiment 1		Experiment 2		Total
	Animals (n = 17)	%	Animals (n = 17)	%	Animals (n = 34)	%
Correct	16	94.1	13	76.5	29	85.3
Incorrect	0	0.0	0	0.0	0	0.0
Unassigned	1	5.9	4	23.5	5	14.7

The seventeen animals were validated based on the ranges found for K/K and K/k+. For experiment 1, the ΔCt range for K/K was 0.68–1.43 and for K/k+ 1.75–2.50. For experiment 2, the ΔCt range for K/K was 0.63–1.24 and for K/k+ 1.50–2.10.

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