Figure 3

Protein sequence comparison among 27 ELAV-like proteins forms. Alternative protein forms are included, specifically for Drosophila RBP9 (A and D) and three of the human proteins (HuB, HuC and HuD, where HuX-n refers to the n amino acid long form of the HuX protein). "*" indicate that amino acids are identical in all 27 sequences, ":" and "." respectively indicate conserved and semi-conserved substitutions. The octamer RNP-1 and the hexamer RNP-2, diagnostic of RRMs, are underlined. Also underlined is a conserved octamer present in the region that is crucial for nuclear export and localization. The regions in light grey boxes have been mapped as necessary for these processes in D. melanogaster ELAV, human HuR and human HuD. We identified eight exon junctions labelled J1 to J8 (see text). Bold characters and dark grey boxes are used to identify amino acids encoded by exon junctions. When the splicing connects intact codons, two amino acids are bold (J3 and J8). The symbol//replaces 85 non-conserved amino acids in the C. elegans sequence.