Figure 1

Diversity of the murine PR130/72 and PR59 isoforms. A: Autoradiography of [³⁵S]-labelled in vitro transcription-translation products of plasmids (1 μg) containing cDNA of murine PR59/B"δ1 (lane δ1), PR59/B"δ2 (lane δ2) and PR59/B"δ3 (lane δ3). B: Immunoprecipitation of mPR72/PR130 isoforms from different murine cells and tissues (N = NIH 3T3, H = heart, B = brain, A = adrenal gland) using pre-immunisation sera (as negative control) and three different PR72/PR130 antibodies: PR72_rec. AB recognizes all putative PR72 isoforms and the PR130 isoforms which contain the common C-terminal region, PR130_{N rec.} AB recognizes all PR130 isoforms, and PR72_{N pept.} AB recognizes all PR72 isoforms.