Figure 10

Cell cycle profile of EGFP, hPR72-EGFP, hPR130-EGFP, hPR70-EGFP, mG5PR/B"γ-EGFP, mPR59/B"δ1-EGFP and mPR59/B"δ2-EGFP expressing HeLa cells. 48 h after transfection with the different expression plasmids, HeLa cells were stained for DNA content (propidium iodide), and the cell cycle profile of EGFP-positive cells was measured. Panel A contains untreated cells whereas cells of panel B were treated with nocodazole (16 h, 1 mg/ml) prior to analysis. Quantification of the percentages of cells in G1, S and G2/M stages of the cell cycle are included. These are results from one typical experiment. Each condition was performed at least 4 times giving similar results but the outcome was highly dependent on the (over)expression level.