Primer | DNA sequence (5' → 3') | Reference or source |
---|---|---|
Construction of Δ so2426 strain | ||
Del840-F | CTTGGTTACCGGCTAGTGAAC | This study |
Del840-R | GGCAGGTATTGATAACAATGA | This study |
Del900-F | GGTTCACACCAATCGCATTAG | This study |
Del900-R | TGGCCAATACCCGCTTACCGC | This study |
ISU2426 | GCCTAAGATGCCATCAGT | This study |
ISD2426 | TCTTCAAGATTTAGCTTATCC | This study |
OP840 | CACATAAGGCAGACCTTCGTC | This study |
OP900 | ATGGTCCGTACTGTGGCCGC | This study |
kanF | ATTGTTGATGCGCTGGCAGT | [32] |
kanR | TCCGGTGAGAATGGCAAAAG | [32] |
Construction of complementation plasmid | ||
2426com-F | ACACACAAGCTTGCGCTTTTCTTTTAGGTACAA | This study |
2426com-R | ACACACGGATCCGACTCACAGAGGGCGCTTA | This study |
RT-PCR analysis | ||
P1 | ATGATATTGATTCTCGTTTG | This study |
P2 | CCGAGTGTGGTTAACTGATG | This study |
P3 | CGCCGAGTATTACTGATATGC | This study |
P4 | AAGCGCTAAAACTGTATCC | This study |
P5 | TTAACATGCATCTACTTTTA | This study |
P6 | GATCTTGCAGGTTGTTGTT | This study |
P7 | TCATACACTCTTTCGCTTAT | This study |
P8 | CTGTTTCTTCAACTCAGCCT | This study |
P9 | CAGTCGTTAGCTCAATTGCT | This study |