Figure 1

Expression of transcripts for hedgehog signaling components Boc and Cdon in adipose depots. A. qPCR assessment of transcript levels in SVF and AD fractions of SC and EP WAT using the Boc primer set. B. qPCR assessment of Boc transcript in whole SC, EP, RP or BAT adipose tissue. C. qPCR assessment of transcript levels in SVF and AD of SC and EP WAT using the Cdon primer set. D. qPCR assessment of Cdon transcript in whole SC, EP, RP or BAT adipose tissue. For A-D, the left panels show data corrected against Gapdh and the right panels show data corrected against 36B4 as internal control for qPCR analysis; values stated in the text are the average of the Gapdh-corrected and 36B4-corrected data for each comparison. In A and B, the level in SC AD was set to a value of 1. SVF, stromal vascular fraction; AD, adipocyte fraction. In B and D, the level in SC WAT was set to a value of 1. For A, * indicates p < 0.001 for EP SVF vs. SC SVF and for EP AD vs. SC AD and # indicates p < 0.001 for EP SVF vs. all others (both panels). For B, * indicates p < 0.001 for EP vs. all others. For C, * indicates p < 0.001 for EP SVF vs. all others (both panels). For D, * indicates p < 0.001 for EP vs. all others (left panel) and EP vs. SC and BAT (right panel).