Expression of Boc and Cdon transcript in wild type and ob/ob tissues. qPCR assessment of transcript level in wild type C57Bl/6J (WT) and ob/ob SC WAT, EP WAT, RP WAT and BAT depots using the Boc primer set (A) and the Cdon primer set (B). For A and B, the left panels show data corrected against Gapdh and the right panels show data corrected against 36B4 as internal control for qPCR analysis; values stated in the text are the average of the Gapdh-corrected and 36B4-corrected data for each comparison. The level in WT BAT was set to a value of 1. For A, * indicates p < 0.001 for comparisons of WT vs. ob/ob samples for each of SC, EP, RP and BAT and # indicates p < 0.005 for ob/ob SC vs. all other ob/ob samples, and **, P < 0.005 for WT EP vs. all other samples (both panels). For B, * indicates p < 0.001 for WT vs. ob/ob BAT WT (both panels) and for WT vs. ob/ob for SC and EP depots left panel only.