Figure 5
Reduced expression of Mup transcript(s) in WAT of ob/ob mice. A. qPCR assessment of transcript level in wild type (WT) and ob/ob (ob) SC WAT and RP WAT depots using the Mup1 primer set. B. qPCR assessment of transcript level in WT and ob SC WAT and RP WAT depots using the Mup1/2 primer set. For each graph, the signal in the respective WT tissue was set to a value of 100. For A and B the left panel shows data corrected against Gapdh and the right panel show data corrected against 36B4 as internal control for qPCR analysis; values stated in the text are the average of the Gapdh-corrected and 36B4-corrected data for each comparison. For A and B, * indicates p < 0.001 for WT SC vs. ob SC and for WT RP vs. ob RP. C. Northern blot analysis of 5 μg of total RNA from the indicated WAT depot or BAT from WT or ob/ob mice. Blot was hybridized to Mup1, aFABP or SCD1 32P dATP-labeled probes. Ethidium bromide staining of rRNA is shown as a gel loading control.