Figure 10
From: Gene organization, evolution and expression of the microtubule-associated protein ASAP (MAP9)
Expression of ASAP in differenciated neural stem cells. (A-D) After 9 days of differentiation neural stem cells differentiate into neurons and astrocytes. (A) Nuclei stained with DAPI (blue), (B) astrocytes revealed by GFAP immunostaining (green), (C) neuronal cells identified by detection of β-III tubulin (red), (D) merged visualization of A-C. (E-H) ASAP is expressed in neurons but not in astrocytes after 9 days of differentiation of neural stem cells. (E) Nuclei stained with DAPI, (F) detection of ASAP, (G) β-III tubulin positive neuronal cells, (H) merged visualization of E-G. Scale Bar: (A-D), 100 μm; (E-H), 50 μm.