Identification of direct regulatory targets of the transcription factor Sox10 based on function and conservation

BMC Genomics

Table 1 The candidate 10 genes and 23 putative Sox10 binding sites tested by ChIP assay.

gene symbol	RefSeq ID	target cis element	chromosome	start	end	Location	primer pair #
Cmkor1	NM_053352	tttATTGTtctc	9	89344237	89344250	Intron	1
		agcACAATgg	9	89346538	89346547	Intron	2
Gda	NM_031776	ttgACAATtt	1	224640748	224640757	Intron	3
PLP	NM_030990	cctATTGTgttt	X	124488088	124488101	5'enhancer	4
		taATTGTttt	X	124492945	124492954	intron	5
		ctATTGTata	X	124493179	124493188	Intron	6
		gaATTGTgtg	X	124493238	124493247	Intron	6
		ggtACAATtg	X	124493249	124493258	Intron	6
		ccATTGTtta	X	124494810	124494819	Intron	7
Sox10	NM_019193	agATTGTcca	7	117150489	117150498	5'enhancer	8
		caATTGTctg	7	117150400	117150409	5'enhancer	8
SOD3	NM_012880	ccATTGTgcc	14	63387776	63387785	5'enhancer	9
Erbb3	NM_017218	tcATTGTggg	7	1874401	1874410	Intron	10
		gacACAATag	7	1874335	1874344	Intron	10
Ptn	NM_017066	caATTGTtgg	4	64147810	64147819	Intron	11
		cacACAATag	4	64147702	64147711	Intron	11
		taATTGTgag	4	64136510	64136519	Intron	12
		atATTGTaac	4	64127690	64127699	Intron	13
		attACAATaa	4	64126376	64126385	Intron	14
Ngfr	NM_012610	tggACAATgg	10	84280255	84280264	Intron	15
		cagACAATgg	10	84280237	84280246	Intron	15
Gzmb	NM_138517	cgATTGTgat	15	35199893	35199902	5'enhancer	16
Gas7	NM_053484	ccATTGTatg	10	54304002	54304011	Intron	17

The number in the last column indicates the primer pair used to test each site by ChIP assay (see additional data file 3).

ISSN: 1471-2164