Figure 1

Features of the BOV program. Screenshots of the BOV program outlining key features of the application. (A) Main page showing input fields for the user's email and single or batch query BLAST output files. The user's email is requested in order to send a separate URL providing access to the BOV output file generated for any given input for 60 days. (B) A summary table of queries, including an individual link to the corresponding hits (indicated by the red arrow), is generated when batch query input files are uploaded. (C) All HSPs for a given query and hit sequences are initially displayed with colored lines connecting the start and end positions of each matching region along the sequence. Individual HSPs can be selected (indicated by the red circle) for in-depth analysis or the list of HSPs can be sorted or filtered to meet certain criteria prior to analysis. (D) The Zoom function can be used to examine a particular region along the hit or query sequence. (E) The Highlight function, activated by clicking on the column (indicated by the red-circle), changes the graphical display from lines (default) to trapezoids. (F) Pair-wise alignments for individual HSPs can be seen by clicking on the Alignment column for an individual HSP region. (G) The individual query or hit sequence region for each HSP can be viewed by clicking on the appropriate button at the top of the pair-wise alignment window.