A combination of LongSAGE with Solexa sequencing is well suited to explore the depth and the complexity of transcriptome

Table 1 Major characteristics of the Sanger_Hypo and Solexa_Hypo LongSAGE libraries

	Sanger_Hypo	Solexa_Hypo
RNA origin	Hypothalamus	Hypothalamus
Mouse strain	Fvb	C57BL/6
RNA amplification	No	Yes
Sequencing method	Sanger	Solexa
Tag length	21 bp	21 bp
Total number of tags	68,023	11,017,712
Number of unique tags	23,007	609,407
Number of tags with only one occurrence	15,612	193,917
% of tags matching to the genome*	90.28	92.25

* We consider that a tag matches to the genome if it has 100% identity over its whole length (21 bp).

ISSN: 1471-2164