Figure 2

Gene expression confirmation of DDRT-PCR and microarray transcripts. Relative mRNA abundance of selected transcripts was confirmed using real time RT-PCR or nothern blot analyses in pigs fed 150, 1,000 or 2,000 mg Zn/kg for 14 d post-weaning. A) PRDX4 mRNA Abundance. Relative real time RT-PCR was performed and fold changes relative to GAPDH and a common Zn₁₅₀ reference sample are presented. Values are means ± SEM, n = 8, (except Zn₁₅₀, n = 7). A significant zinc effect was detected for relative PRDX4 mRNA abundance, P < 0.009. No Zn by phytase interaction, or phytase effect were detected. B) GLO1 mRNA Abundance. Northern blot analysis was performed and values are mean optical density readings ± SEM, n = 8 (except Zn₁₅₀, n = 7). A significant zinc effect was detected for relative hepatic GLO1 mRNA abundance, P < 0.0007. No Zn by phytase interaction, or phytase effect were detected. C) ACY1 mRNA Abundance. Northern blot analysis was performed and values are mean optical density readings ± SEM, n = 8 (except Zn₁₅₀, n = 7). A significant zinc effect was detected for relative hepatic ACY1 mRNA abundance, P < 0.01. No Zn by phytase interaction, or phytase effect were detected. D) HSP70.2 mRNA Abundance. Relative real time RT-PCR was performed and fold changes relative to Zn₂₀₀₀ using 18S as normalizing gene are presented. Values are means ± SEM, n = 7, (except Zn₂₀₀₀, n = 8). A significant zinc effect was detected for relative HSP70.2 mRNA abundance, P < 0.002. No Zn by phytase interaction, or phytase effect were detected.