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Table 1 List of differentially expressed products and sequence identity information from differential display reverse transcription PCR.

From: Gene expression profiling in hepatic tissue of newly weaned pigs fed pharmacological zinc and phytase supplemented diets

EST ID (Length – bp)

Sequence IDa

Species/Accession No.b

Porcine TC numberc

Pattern of transcript abundanced

Signal Transduction Genes

CF106654 (152)

Activator heat shock 90 kDa protein ATPase homologue 1 (AHSA1)

B. taurus/NM_001034666.1

TC243227

3 = 4 > 2 > 1

CF106663 (151)

Activator heat shock 90 kDa protein ATPase homologue 1 (AHSA1)

B. taurus/NM_001034666.1

TC243227

3 = 4 > 2 > 1

CF106649 (240)

Golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal) 1, (GOLGB1)

H. sapiens/NM_004487.3

TC269106

1 > 2 = 3 = 4

CF106669 (441)

Interferon induced with helicase C domain 1 (IFIH1)

H. sapiens/NM_022168.2

TC281891

3 > 4 > 2 > 1

CB826605 (152)e

N-myc downstream regulated gene 1 (NDRG1)

B. taurus/NM_001035009.1

TC257462

1 > 4

CB826606 (130)e

Orosomucoid 1 (ORM1)

S. scrofa/PIGA1AG

TC273225

1 > 4

CF106636 (484)

Orosomucoid 1 (ORM1)

S. scrofa/PIGA1AG

TC273225

4 > 3 > 2 = 1

CF106686 (546)

Orosomucoid 1 (ORM1)

S. scrofa/PIGA1AG

TC273225

3 = 4 > 2 = 1

CF106640 (240)

Progesterone receptor membrane component 1 (PGRMC1)

S. scrofa/NM_213911.1

TC238451

4 = 3 > 2 > 1

CF106680 (272)

Progestin and adipoQ receptor family member VII (PAQR7)

S. scrofa/NM_213739.1

TC238374

3 > 4 > 2 = 1

CF106679 (354)

Ras, member oncogene family (RAB2A)

C. familiaris/NM_001003318.1

TC241036

4 = 3 > 2 > 1

CF106667 (620)

Ribosomal protein L17 (RPL17)

B. taurus/NM_001034459.1

TC264931

4 > 3 > 2 > 1

CF106653 (407)

Ribosomal protein SA (RPSA)

S. scrofa/AM050292.2

TC269871

3 > 4 = 2 > 1

DW177048 (174)

Tight junction protein 1 (zona occludens 1, TJP1)

C. familiaris/NM_001003140.1

TC281469

2 = 3 > 1

CF106648 (228)

Tyrosine 3-monooxygenase/Tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB)

H. sapiens/NM_139323.2

TC251407

3 = 4 = 2 > 1

CF106651 (310)

Wilms' tumour associating protein (WTAP)

H. sapiens/NM_004906.3

TC267553

4 = 3 > 2 > 1

Nucleic Acid Metabolism Genes

CF106652 (492)

Basic Transcription Factor 3 (BTF3)

H. sapiens/NM_001207.4

TC241093

4 = 2 > 3 > 1

CF106681 (396)

DNA Polymerase Beta (POLB)

B. taurus/NM_001034764.1

TC252856

4 = 1 > 3 > 2

CF106666 (623)

Heterogeneous nuclear ribonucleoprotein K (HNRPK)

H. sapiens/NM_002140.2

TC260259

4 = 3 = 2 > 1

CF106673 (385)

La ribonucleoprotein family, member 4 (LARP4)

H. sapiens/NM_199190.1

TC244218

3 > 2 > 1 > 4

Oxidative Stress Genes

CF106641 (364)

Flavin containing monooxygenase 3 (FMO3)

B. taurus/NM_174057.2

TC251612

1 > 2 = 3 = 4

CF106665 (479)

Glyoxalase I (GLO1)

B. taurus/NM_001083496.1

TC254694

4 > 3 > 2 > 1

CF106659 (349)

Peroxiredoxin (PRDX4)

B. taurus/NM_174433.2

TC239253

4 = 3 > 2 > 1

Blood Cogulation Genes

CF106687 (278)

Carboxypeptidase B2 (CPB2)

H. sapiens/NM_001872.3

TC240093

3 = 4 > 2 = 1

CF106639 (374)

Coagulation Factor IX (F9)

H. sapiens/NM_000133.2

TC286008

4 > 2 > 3 = 1

CF106662 (269)

Histidine Rich Glycoprotein (HRG)

B. taurus/NM_173919.2

TC251128

3 > 4 > 2 = 1

Protein and Amino Acid Metabolism Genes

CF106650 (196)

N-Aminoacylase I (ACY-1)

S. scrofa/NM_213896.1

TC269425

4 = 3 = 2 > 1

CF106684 (295)

Polyubiquitin (UBB)

B. taurus/NM_174133.2

TC260685

3 = 4 > 2 = 1

Structural Genes

CF106657 (384)

Integrin alpha-6 (ITGA6)

H. sapiens/NM_000210.2

TC285515

1 > 2 = 3 = 4

Mitochondrial DNA Genes

CF106643 (300)

Mitochondrial DNA

S. scrofa/AB292606.1

TC259884

1 > 3 > 2 = 4

CF106645 (177)

Mitochondrial DNA

S. scrofa/AB292606.1

TC259884

1 > 2 > 3 = 4

CF106671 (297)

Mitochondrial DNA

S. scrofa/AB292606.1

TC259884

4 = 3 > 2 > 1

CF106646 (156)

Mitochondrial DNA

S. scrofa/AB292606.1

SINGLETON

3 > 4 > 2 = 1

CF106664 (336)

Mitochondrial DNA

S. scrofa/AB292606.1

TC270954

1 > 2 > 3 = 4

CF106675 (334)

Mitochondrial DNA

S. scrofa/AB292606.1

TC270954

3 > 4 = 2 = 1

CF106647 (207)

Mitochondrial DNA

S. scrofa/AB292606.1

TC239708

3 > 4 = 2 = 1

Unknown Identity Genes

CB826607 (112)e

No significant match

 

SINGLETON

1 > 4

CB826608 (288)e

No significant match

 

TC292722

1 > 4

CB826609 (250)e

No significant match

 

SINGLETON

1 > 4

CB826610 (147)e

No significant match

 

TC263361

1 > 4

CF106637 (262)

No significant match

 

TC269532

4 = 3 > 2 > 1

CF106638 (366)

No significant match

 

SINGLETON

4 = 2 > 2 > 1

CF106642 (557)

No significant match

 

SINGLETON

1 > 2 = 4 > 3

CF106644 (312)

No significant match

 

SINGLETON

1 > 2 = 3 = 4

CF106655 (211)

No significant match

 

SINGLETON

3 = 4 > 2 > 1

CF106656 (222)

No significant match

 

SINGLETON

1 = 3 > 2 > 4

CF106658 (294)

No significant match

 

TC288854

4 = 2 > 3 = 1

CF106660 (209)

No significant match

 

SINGLETON

1 > 4 = 3 > 2

CF106661 (228)

No significant match

 

SINGLETON

1 = 2 > 3 > 4

CF106668 (223)

No significant match

 

SINGLETON

1 > 2 = 3 = 4

CF106670 (225)

No significant match

 

TC264413

1 = 3 > 4 > 2

CF106672 (372)

No significant match

 

TC275435

3 > 2 > 1 > 4

CF106674 (536)

No significant match

 

SINGLETON

3 > 1 = 2 = 4

CF106676 (225)

No significant match

 

TC264413

3 = 1 > 4 > 2

CF106678 (434)

No significant match

 

TC284960

3 > 4 = 2 = 1

CF106682 (317)

No significant match

 

TC264213

3 > 4 > 2 = 1

CF106683 (364)

No significant match

 

TC256701

3 > 4 > 2 = 1

CF106685 (227)

No significant match

 

SINGLETON

1 = 4 > 2 < 3

DW177047 (247)

No significant match

 

TC241849

3 > 1 = 2

DW177049 (158)

No significant match

 

SINGLETON

2 > 1 = 3

DW177050 (133)

No significant match

 

TC248509

1 = 2 > 3

  1. aAmplicons were compared to GenBank database using the BLASTN software v. 2.2.17 August 26, 2007.
  2. bSpecies, GenBank accession number for most significant match.
  3. cAmplicons were compared to TIGR Sus scrofa gene index v.12.0 updated June 20, 2006 using the BLAST software v. 2.0. Singleton denotes sequences unassigned to a TC.
  4. dPattern of transcript abundance observed on DDRT-PCR gels. 1 = Zn150; 2 = Zn1000; 3 = Zn1000P; 4 = Zn2000. Pattern observed in the display gel from highest (>) to lowest or comparable (=) abundance.
  5. eAmplicons detected in Exp. 1.
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