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Figure 2 | BMC Genomics

Figure 2

From: Global identification of bursicon-regulated genes in Drosophila melanogaster

Figure 2

Temporal response of the ligated flies to the r-bursicon heterodimer. Newly emerged flies were ligated between the head and thorax at emergence. These flies with unsclerotized cuticle at 1 h after neck-ligation were injected with 0.5 μl of the purified r-bursicon α (negative control) or r-bursicon heterodimer expressed in mammalian HEK293 cells. The cuticle sclerotization process was photographed at the indicated time periods after injection. The arrow indicates the area with the unsclerotized cuticle (light color) in control and the sclerotized cuticle (darkened) in the animals injected with r-bursicon heterodimer.

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