Figure 1

Alignment and phylogenetic analysis of G-protein alpha subunits. The H. jecorina genome comprises 3 G-alpha subunits belonging to the three subgroups common in other fungi. Accession numbers (corresponding database: GenBank) of the protein sequences used are those of the nearest neighbours of Trichoderma reesei (Hypocrea jecorina) (TR) in Saccharomyces cerevisiae (SC), Schizosaccharomyces pombe (SP), Neurospora crassa (NC), Aspergillus fumigatus (AF), Gibberella zeae (GZ), Fusarium oxysporum (FO), Magnaporthe grisea (MG), Cryphonectria parasitica (CP), Rattus norvegicus (RN), Canis familiaris (CF), Drosophila melaongaster (DM), Oryza sativa (OS), Pisum sativum (PS) and Glycine max (GM), are as listed in Table 1. Accession numbers (corresponding database: GenBank) of the G-protein alpha subunits of Ustilago maydis (UM) are: GPA1 P87032, GPA2 P87033, GPA3 P87034 and GPA4 P87035. (A) Alignment of GTP-binding domains and GTPase domains of G-protein alpha subunits including the two additional putative G-protein alpha subunits of H. jecorina (tre43177 and tre38187). While the GTPase domain of these two proteins matches the consensus (DXXGQ), the GTP binding domain is altered at the same position as in U. maydis Gpr4p (GXG XXGKS/T) [32]. (B) Phylogenetic analysis of G-alpha subunits using the Minimum evolution method and 500 Bootstrap replications as test of phylogeny.