MLST analysis of carrier and non-carrier strains reveal no lineage specific differences. The dendrogram shows the various lineages of S. aureus tested by MLST. In all seven genes tested for MLST, D30 (double asterisk) and 930918-3 (single asterisk) were identical. Interestingly, strains D30 and 930918-3 belong to the same clonal complex as strain USA300 and NCTC8325. It can also be seen that MRSA252 is similar to the carrier strains D39 and D20. Strain 502-A, which is capable of experimentally induced intermittent nasal carriage, was seen to belong to the same clonal complex as strains Mu50 and N315 (found in the same clade in the dendrogram) and the carrier strain D94. Such varied clustering of carrier strains shows nasal carriage is not dependent on vertical lineage of the strains.