Figure 5

Real-time quantitative RT-PCR expression of meiosis-specific transcripts. Quantification of TEX11 and DMC1 gene expression in sheep ovaries at the stages indicated (D44, 55, 60, 65, 75, 82, 90, 114 and adult). HPRT1 (hypoxanthine phosphoribosyltransferase) gene was used as a reporter gene. For both genes, a peak was observed at around 60 dpc then a decrease as from 75 dpc. No expression was detected in adult ovary. In males, levels of transcription were very low during fetal life. By contrast, strong expression was observed in adult testis. SEM were calculated at stages D60, 82, 90 and adult.