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Figure 1 | BMC Genomics

Figure 1

From: A novel reverse transduction adenoviral array for the functional analysis of shRNA libraries

Figure 1

Immobilization of viral spots in a microarray format. A. Adenovirus encoding shRNAs directed against different target genes (1, 2, 3, ..., n) are immobilized on a glass slide. B. Coomassie staining of HeLa cells seeded on the adenoviral microarray. Due to the thickness of the carrier (glass-slide), shadows of the printed spots were cast on the stage plate of the microscope. This "shadowing" is not observed by using conventional fluorescence microscope, used for image analysis. Scale bar, 100 μm. C. Neighbouring spots of U-2 OS cells on the microarray. The cells were alternately infected with two types of adenoviruses, encoding either enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP), respectively. Scale bar, 100 μm. D. Quantitative analysis of viral spots, eight spots for each fluorescence marker, from Figure 1C for the detection of intermixture between neighbouring viral spots. E. Infection efficiency of U-2 OS cells: Percentage of the number of cells expressing EGFP compared to total number of cells as determined via DAPI stain (4 spots, n > 150).

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