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Figure 4 | BMC Genomics

Figure 4

From: Evolution of the holozoan ribosome biogenesis regulon

Figure 4

Frequency of E(CG) in opisthokont RiBi promoters. The frequency of E(CG) in 25 opisthokont RiBi orthologs was investigated. These 25 RiBi orthologs were selected based on the presence of conserved E(CG) sites in the human, fly, sea anemone, and choanoflagellate orthologs (Table 1B). DNA sequences 500 bp upstream from the translational start sites in the RiBi orthologs of S. cerevisiae (Sc), C. glabrata (Cg), K. lactis (Kl), A. gossypii (Ag), P. stipitis (Ps), D. hansenii (Dh), C. albicans (Ca), Y. lipolytica (Yl), N. crassa (Nc), S. pombe (Sp), and M. brevicollis (Mb) were collected. For D. melanogaster (Dm), 500 bp of DNA sequence (± 250 bp) from the 5' annotated end was collected. The sequences of each of these opisthokont promoters was analyzed for the presence of E(CG) motifs. The percentage of (ECG) in each species' RiBi orthologs is depicted on the Y-axis with the number of orthologs containing E(CG) over the total ortholog number of orthologs displayed above each genome. Key nodes for latest common ancestors (LCAs) are depicted in the phylogenetic tree [5961].

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