Antigen stimulation of peripheral blood mononuclear cells from Mycobacterium bovis infected cattle yields evidence for a novel gene expression program

Table 4 Real time qRT-PCR primer sequences, optimum primer concentrations and amplicon sizes for all validated genes.

Gene symbol	Forward primer (5'-3')	Reverse primer (5'-3')	Amplicon size (bp)	Primer conc. (nM)
AKT1	GGTGATCCTGGTGAAGGAGA	GAGTACTTCAGGGCCGTCAG	159	900
CHUK	CCGGAAGCTACTCAACAAACCA	CATGCAAATATCGGATCCCAG	101	900
H3F3A	CATGGCTCGTACAAAGCAGA	ACCAGGCCTGTAACGATGAG	136	100
IFNG	TGATGGCATGTCAGACAGCA	GGCACAAGTCATATAGCCTGACAC	51	300
IKBKB	CCTGAAGATTGCGTGTAGCA	ACTCTGGTCCTGCTCCTTCA	229	300
IL10	CTTGTCGGAAATGATCCAGTTTT	TCAGGCCCGTGGTTCTCA	66	300
IL1A	GCTATGAGCCACTTCGTGAGGA	TGCCACCATCACCACATTCTC	110	300
IL1R1	GAATCCTTTAAACAGAAGAA	TGGATGTATTAGTTGTATGTAT	145	300
IL1R2	CCTGTGATCATCTCTTCCCACC	GCAGAGTGGTTGTGTGTATGCC	106	300
IL1RN	CCCCACAACCCTTTCATCAA	GGTCAGGAGAAGCCACATTTG	68	300
IL8	AGGTGGTGTTTGAAGCCCAT	CACAACCTTCTGCACCCACTT	123	900
IRAK1	TCAGCGACTGGACATCCTTCT	GGACGTTGGAACTCTTGACATC	101	300
LTA	CCGAGGAGGACTCAGAAACTGA	ACGCCTCTTCTTTCTTCGCCT	111	300
MAPAPK2	GGACGTCAAGGAGGAGATGA	CTTCAGAAGCAGAGGGTTGG	106	300
MYD88	TGCCTCTGTGTGCCTGTACATC	AGATATGGACCATGGCTGCA	134	300
NCOR1	CCTGTGAGAACGAAAACATCAAAC	TTGAGCCTGGTCTCTGATGGT	79	900
NFKB1	ATACTGAACAATGCCTTCCGG	CACGTCAATGGCCTCAGTGTAG	135	300
TGFB1	TGCTTCAGCTCCACAGAAAAGA	AGGCAGAAATTGGCGTGGT	116	300
TICAM2	TGGAGAAGACCCACCTTTGTTT	TAGATCCTCAGCTCTGCTTCGG	163	300
TLR2	CCATTGACAAGAAGGCCAT	AACCCTTCCTGCTGAGTCTCAT	106	900
TLR4	CGAGAGCACCTATGATGCCTTT	ATGGCCACCCCAGGAATAAA	144	900
TNF	TCTACCAGGGAGGAGTCTTCCA	GTCCGGCAGGTTGATCTCA	68	300
TOLLIP	AAATGAGAACACAGTGCGCTCT	CATCCCATTAAGCCTACGTGG	186	300

ISSN: 1471-2164