Figure 1
From: Selection and mutation on microRNA target sequences during rice evolution
Flow chart for the methods used in this study. Two approaches, molecular evolution and population genetics were used to analyze a selection of the miRNA target genes and the gain or loss of miRNA binding sites. a: the rice genome annotation by the TIGR; b: empirical parameters: no mismatch at positions 10 and 11; no more than one mismatch at positions 2–12; no more than two consecutive mismatches downstream of position 13; c: 76 miRNAs from miRBase (Release 11.0, http://microrna.sanger.ac.uk) and 38 newly identified miRNAs [3]; d: see Additional file 8; e: see Additional file 4.