Figure 5
Expression of the pmr locus is reduced by adrenaline. Expression of the pmrHFIJKLM operon after a 30 minute exposure to adrenaline (50 μM) was assessed in SL1344pM by measuring luminescence per OD600 as described in "Methods". The significant transcriptional reduction (10%; P ≤ 0.05) in expression was fully reversed by addition of β-adrenergic blocker propranolol (500 μM). Luminescence is expressed as a percentage of the water addition control in relative light units per culture optical density (RLU/OD600). Experiments were repeated at least three times. Asterisk indicates significant difference by the student t-test. Standard error bars are shown. Water, H2O; Adrenaline, ADR; Propranolol, PO.