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Figure 1 | BMC Genomics

Figure 1

From: A MITE-based genotyping method to reveal hundreds of DNA polymorphisms in an animal genome after a few generations of artificial selection

Figure 1

Schematic illustration of the DArT protocol. (A) Principle of the MITE-based genome complexity reduction method. Genomic DNA is digested by restriction enzyme Bsp1286I, and Bsp1286I adaptors are ligated to the generated overhangs. Then two rounds of PCR amplifications are performed using two primers: one annealing to Bsp1286I adaptors (Bsp1286I primer), and one complementary to a conserved sequence motif of the Pony element. For the most part, the resulting genomic representations include fragments with the Bsp1286I restriction site on one extremity and the Pony motif on the other one, because the PCR conditions are adjusted to preferably amplify this particular type of fragments. (B) Principle of the polymorphism detection on DArT microarrays. Genomic representations of each sample are hybridized against a library containing all fragments spotted on a slide. When a fragment is missing in one representation, it will not hybridize to the corresponding fragment on the slide. In this example, monomorphic fragments present in both representations are scored as '-' while polymorphic fragments present or absent in one representation are scored as '1' or '0', respectively.

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