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Table 1 Adaptor and primer sequences used for preparation of genomic representations and library construction

From: A MITE-based genotyping method to reveal hundreds of DNA polymorphisms in an animal genome after a few generations of artificial selection

 

Adaptors (5'-3')

Primers (5'-3')

Bsp1286I restriction site

Preparation of genomic representations for genotyping

Bsp1286I adaptors I

Bsp1286I primer I

 

• Forward strand: equimolar mix of CATAGGTGTCCACAGTCGGTCTGCA CATAGGTGTCCACAGTCGGTCTGCT CATAGGTGTCCACAGTCGGTCTGCC

AGGTGTCCACAGTCGGTCT

 

• Reverse strand GACCGACTGTGGAC

 

Library construction

Bsp1286I adaptors II

Bsp1286I primer II

 

• Forward strand: equimolar mix of CTGAGTAGTGCCAGAACGGTCTGCA CTGAGTAGTGCCAGAACGGTCTGCC CTGAGTAGTGCCAGAACGGTCTGCT

CTGAGTAGTGCCAGAACGGTC

 

• Reverse strand GACCGTTCTGGCA

 

Pony sequence

-

1536-clone preliminary library construction

  

PonyAll primer

  

CGNAATNTGARYCAAAACGGTA

  

4608-clone expanded library construction and preparation of genomic representations for genotyping

  

PonyB primer

  

GGANGCGTATTCTTYACCCAC