Table 2 Comparison of the differential expression ratios observed between embryonic stages using amplified and unamplified material. The expression ratios observed with amplified targets (IVT and PCR) come from the array datasets presented in this study. Those originating from Real-Time PCR come from the gene specific validations (IFN-tau, Cox2) we performed on unamplified RNA from each stage. Those originating from Northern blots (c12, c93, PAG11) come from previous results on unamplified RNA from each stage [26].
From: Amplification biases: possible differences among deviating gene expressions
Array | Northern | Real-time PCR | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
IVT amplified material | PCR amplified material | (mRNA/18S) | (mRNA/Gapdh) | |||||||||
Gene | ov/tub | tub/fil | ov/fil | ov/tub | tub/fil | ov/fil | ov/tub | tub/fil | ov/fil | ov/tub | tub/fil | ov/fil |
IFN-tau | -3,21 | -1,57 | -6,94 | -2,4 | -2,31 | -5,55 | -5,48 | -1,57 | -8,63 | |||
Cox2 | -1,72 | -1,26 | -2,17 | -1,22 | -1,73 | -2,10 | -4,32 | -1,74 | -7,53 | |||
c12 | 4,93 | 3,86 | 8,51 | 1,87 | -1,26 | 1,48 | 3,24 | 2,63 | 4,02 | |||
c93 | -1,2 | -1,83 | -2,19 | -1,66 | -1,62 | -2,67 | -1,52 | -2,87 | -4,37 | |||
PAG11 | -1,52 | -2,87 | -4,37 | -1,49 | -1,06 | -1,58 | -2,98 | -1,3 | -3,87 | |||