Toxicogenomic response of Pseudomonas aeruginosa to ortho-phenylphenol

BMC Genomics

Table 2 Transcript level comparison of P. aeruginosa genes between real-time PCR and microarray analyses

Gene	^amRNA level change with microarray		^bmRNA level change with real-time PCR		Forward primer sequence (5'-3')	Reverse primer sequence (5'-3')
	Fold change		Fold change
	20 min	60 min	20 min	60 min
PA4243	2.914	6.799	3.605 (± 1.55)	7.378 (± 0.7)	ATGGCTAAGCAAGGTGCTCTCTCT	ACGATGATCGCCAGGAACAGGAAA
PA2193	-2.268		-1.967 (± 1.06)		TGAACGTCAACACGATATCCAGCC	ATTGAGCACGTTGAGCACGGTCT
PA1173		-3.077		-3.03 (± 5.64)	ATCGACAAGGACAGCAACAAGTGC	GTCCATGTAGTGGGTGATGCTGAT
PA3049	-6.25	-25.907	-10.196 (± 0.14)	-48.503 (± 0.35)	TCGTGATCTTTGTCCGTTCACCCA	CGTGCTGGAGTTGATTGAGACGTT
PA3724	-2.762	-10.256	-5.856 (± 0.21)	-14.928 (± 1.27)	TCATCACCGTCGACATGAACAGCA	AGTCCCGGTACAGTTTGAACACCA
PA3622	-2.653	-2.967	-1.954 (± 1.95)	-5.924 (± 1.60)	TGACCACGATGATGAAGTGCTCCT	TTGGAAGAGAAGGAAGTGGTGGCT
^cPA3001	1.00	1.00	1.00	1.00	GCACCATCACCATCGACGAAGAAA	TCTTGATGCCGTACTGGGTGTAGT

The real time PCR results are the mean of three biological replicates with three technical replicates for each gene. The microarray results are the mean of four replicates of each gene.
^aThe microarray results are the mean of four replicates of each gene.
^bThe real time PCR results are the mean of three biological replicates with three technical replicates for each gene.
^cInternal control: glyceraldehyde-3-phosphate dehydrogenase

ISSN: 1471-2164