Figure 2
From: High-throughput cell-based screening reveals a role for ZNF131 as a repressor of ERalpha signaling
Electrophoretic mobility shift assay: ZNF131 suppresses ERα-ERE binding activity. EMSA of ERα-ERE binding activity using nuclear protein from: (lanes 2 and 3) control vector pcDB-transfected cells in the presence of 10 or 100 nM E2; (lanes 4 and 5) ZNF131-transfected cells in the presence of 10 or 100 nM E2; (lane 6) pcDB-transfected cells in the presence of 100 nM E2 where the biotin-labeled ERE probe was competed with 200-fold molar excess of unlabeled ERE probe; and (lane 1) blank control. Results shown are representative of three independent experiments.