ZNF131 suppressed pS2 gene expression at both the protein and mRNA levels. (A) Western blot analysis (top) for pS2 and exogenous ERα expression in HeLa cells transfected with the empty vector (pcDB), ZNF131, or BRCA1, and treated with either E2 (10 nM) or the ethanol vehicle for 12 h. Relative band intensity is shown below. (B) Relative induction of pS2 mRNA in HeLa cells transfected with either pcDB or ZNF131, in the presence (+) or absence (-) of E2. Values are expressed relative to control cultures and represent the mean ± SD of three experiments (*, different from control values; P < 0.05). (C) Western blot analysis of pS2 expression in MCF-7 cells transfected with pcDB, ZNF131 or BRCA1, and treated with either E2 (10 nM) or the ethanol vehicle for 12 h. Relative band intensity analysis using Bio-Rad Quantity One software is shown below. Relative band density values varied, but were qualitatively similar across three independent experiments.