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Table 3 Validation of microarray-based mRNA levels by qRT-PCR

From: Global analysis of gene expression changes during retinoic acid-induced growth arrest and differentiation of melanoma: comparison to differentially expressed genes in melanocytes vs melanoma

   Microarray qRT-PCR
Gene Accession Number B16 48 h RA vs. control melan-a vs. B16 B16 48 h RA vs. control melan-a vs. B16
BRCA2 NM_009765 0.30 0.36 0.301 0.0938
CDC2A NM_007659 0.40 0.22 0.298 0.0311
TOP2A NM_011623 0.50 0.11 0.226 0.00668
TINAGL NM_023476 3.70 4.00 1.81 5.64
PLAT NM_008872 2.10 3.33 3.15 1.43*
DHRS3 NM_011303 4.60 2.70 4.89 133
FGF1 NM_010197 2.20 2.27 3.53 2.35
  1. All qRT-PCR values were significant by Bonferroni-corrected (n = 14) T-test with p < 0.01 with the exception of starred (*) value.