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Table 3 Validation of microarray-based mRNA levels by qRT-PCR

From: Global analysis of gene expression changes during retinoic acid-induced growth arrest and differentiation of melanoma: comparison to differentially expressed genes in melanocytes vs melanoma

  

Microarray

qRT-PCR

Gene

Accession Number

B16 48 h RA vs. control

melan-a vs. B16

B16 48 h RA vs. control

melan-a vs. B16

BRCA2

NM_009765

0.30

0.36

0.301

0.0938

CDC2A

NM_007659

0.40

0.22

0.298

0.0311

TOP2A

NM_011623

0.50

0.11

0.226

0.00668

TINAGL

NM_023476

3.70

4.00

1.81

5.64

PLAT

NM_008872

2.10

3.33

3.15

1.43*

DHRS3

NM_011303

4.60

2.70

4.89

133

FGF1

NM_010197

2.20

2.27

3.53

2.35

  1. All qRT-PCR values were significant by Bonferroni-corrected (n = 14) T-test with p < 0.01 with the exception of starred (*) value.
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BMC Genomics

ISSN: 1471-2164

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