Figure 5

Differential expression pattern of proteins in iDCs treated with [BF/S+L/Ep] and derived phytocompounds. Human iDCs in culture were treated with 0.1% DMSO (vehicle control), [BF/S+L/Ep] (75 μg/ml) and cichoric acid (50 μg/ml) for 12 or 24 h. Aliquots of equal amounts of solubilized total proteins from various test cell samples were analyzed by 2-D gel electrophoresis and MALDI-TOF-MS. Approximately 1,300 protein spots were detected in iDCs. Seventy gels were used for the present study, and highly similar results were obtained from three independent experiments, unless indicated otherwise.