Figure 7
Confirmation of the 2-D gel proteomic results on up-regulation of Mn-SOD protein expression with Western blot analysis. (A) Differential expression of Mn-SOD protein in iDCs treated with [BF/S+L/Ep] (75 μg/ml), cichoric acid (50 μg/ml) or vehicle control, at both 12 and 24 h post-treatment, as revealed by 2-D gel electrophoresis, SYPRO Ruby dye staining, and quantification by PDQuest analysis. The arrows indicate the Mn-SOD protein spots detected in different treatments. The expression ratio compared to control gel is shown in black. (B) Western blot analysis of expression of Mn-SOD and actin in human iDCs treated with phytocompounds. Total cellular proteins were extracted and separated by 10% SDS-PAGE, and Western blot analysis performed with anti-Mn-SOD or anti-actin antibodies. Actin was used as a sample-loading control. Similar results were obtained from three independent experiments.