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Figure 3 | BMC Genomics

Figure 3

From: Improved coverage of cDNA-AFLP by sequential digestion of immobilized cDNA

Figure 3

Effect of the number of PCR products on the fraction of analyzable fragments. For RefSeq EST data sets (Tab. 1), randomly selected enzyme combinations were used to simulate cDNA-AFLP with the sequential digestion. One to three selective nucleotides were attached to PCR primers. For each PCR reaction, the fraction of analyzable fragments (fragments between 40 bp and 700 bp with a length that occurred only once, at least 20 products per PCR) was plotted against the number of PCR products. Vertical bars indicate standard error. Black: Arabidopsis; red: mouse; green: human.

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