Mapped (A) and predicted (B) promoter regions of CysR-controlled genes. Transcription start sites (bold, marked with an arrow and +1) were mapped using the RACE method. The -10 and -35 elements were then inferred either from this transcription start site (bold black) or the location of either McbR  or CysR binding sites (grey elements), based on the published consensus sequences determined by Patek et al. . The potential CysR binding sites are boxed, the inverted repeat is highlighted in light green with matching bases marked with asterisks. Coloured lines mark the regions cloned into pRIM2 for in vivo promoter tests, bases mutated by transition are indicted with M.