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Table 2 Bacterial strains and plasmids

From: The dual transcriptional regulator CysR in Corynebacterium glutamicum ATCC 13032 controls a subset of genes of the McbR regulon in response to the availability of sulphide acceptor molecules

Name Relevant genotype/informationa Source/reference
E. coli DH5αMCR F- endA1 supE44 mcrA thi-1 hsdR17λ- recA1 relA1 Δ(lacZYA-argF) U169 (Φ80dlacZ ΔM15) gyrA96 deoR Δ(mrr-hsdRMS-mcrBC) [58]
ER2566 F- λ- fhuA2 [lon] ompT lacZ::T7 gene1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm] New England Biolabs
C. glutamicum   
ATCC 13032 Wild type, NxR ATCCb
CR030 ΔcysR this study
CR031 mcbR ΔHTH ssuR ΔHTH cysR const this study
CR032 mcbR ΔHTH ssuR ΔHTH cysR ΔHTH this study
CR031-035i CR031 with integrated pCR035i, KmR this study
CR031-036i CR031 with integrated pCR036i, KmR this study
CR031-037i CR031 with integrated pCR037i, KmR this study
CR031-038i CR031 with integrated pCR038i, KmR this study
CR031-039i CR031 with integrated pCR039i, KmR this study
CR031-040i CR031 with integrated pCR040i, KmR this study
CR031-041i CR031 with integrated pCR041i, KmR this study
CR031-042i CR031 with integrated pCR042i, KmR this study
CR031-043i CR031 with integrated pCR043i, KmR this study
CR031-044i CR031 with integrated pCR044i, KmR this study
CR031-045i CR031 with integrated pCR045i, KmR this study
CR031-046i CR031 with integrated pCR046i, KmR this study
CR031-047i CR031 with integrated pCR047i, KmR this study
CR031-048i CR031 with integrated pCR048i, KmR this study
CR032-035i CR032 with integrated pCR035i, KmR this study
CR032-036i CR032 with integrated pCR036i, KmR this study
CR032-037i CR032 with integrated pCR037i, KmR this study
CR032-038i CR032 with integrated pCR038i, KmR this study
CR032-039i CR032 with integrated pCR039i, KmR this study
CR032-040i CR032 with integrated pCR040i, KmR this study
CR032-041i CR032 with integrated pCR041i, KmR this study
CR032-042i CR032 with integrated pCR042i, KmR this study
CR032-043i CR032 with integrated pCR043i, KmR this study
CR032-044i CR032 with integrated pCR044i, KmR this study
CR032-045i CR032 with integrated pCR045i, KmR this study
CR032-046i CR032 with integrated pCR046i, KmR this study
CR032-047i CR032 with integrated pCR047i, KmR this study
CR032-048i CR032 with integrated pCR048i, KmR this study
Plasmids   
pK18mobsacB sacB, lacZa, KmR, mcs [56]
pRIM2 promoterless cat, KmR, dppc [31]
pTYB1 ApR, carrying an intein-coupled chitin binding domain New England Biolabs
pCR030d pK18mobsacB carrying cysR delc this study
pCR031d pK18mobsacB carrying ssuR HTHdel this study
pCR032d pK18mobsacB carrying cysR HTHdel this study
pCR033d pK18mobsacB carrying mcbR HTHdel this study
pCR034d pK18mobsacB carrying cysR constd this study
pCR035i pRIM2 carrying PcysI-A this study
pCR036i pRIM2 carrying PcysI-B this study
pCR037i pRIM2 carrying PcysI-Am5 this study
pCR038i pRIM2 carrying PcysI-Am3 this study
pCR039i pRIM2 carrying Pfpr 2-A this study
pCR040i pRIM2 carrying Pfpr 2-B this study
pCR041i pRIM2 carrying PssuR-A this study
pCR042i pRIM2 carrying PssuR-B this study
pCR043i pRIM2 carrying Pcg 3372-A this study
pCR044i pRIM2 carrying Pcg 3372-B this study
pCR045i pRIM2 carrying Pcg 2810-A this study
pCR046i pRIM2 carrying Pcg 2810-A1m this study
pCR047i pRIM2 carrying Pcg 2810-A2m this study
pCR048i pRIM2 carrying Pcg 2810-A1m2m this study
pCR050 pTYB1 carrying a cysR-intein fusion with a C-terminal glycine this study
  1. a r superscript indicates resistance. Nx, Nalidixic acid; Km, Kanamycin
  2. b ATCC; American Type Culture Collection, Rockville, MD
  3. c the postfix del indicates inserts used for targeted gene deletion.
  4. d the postfix const indicates inserts used for promoter replacement