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Figure 1 | BMC Genomics

Figure 1

From: A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis)

Figure 1

Clone selection and complete insert sequencing of 6,464 Sitka spruce FLcDNAs. A total of 20,469 candidate FL transcripts were identified in two consecutive rounds of clone selection involving initially 32,980 and then 46,745 putative unique transcripts (PUTs) derived from a total of 147,146 high-quality 3'-end ESTs. See Methods for complete details of candidate clone selection criteria. Among the 8,127 candidates selected for complete insert sequencing, 5,298 were finished by end reads only, and another 1,166 were finished by end reads plus gap closing using primer walking, yielding a total of 6,464 sequence-verified finished FLcDNAs. An additional 1,396 clones (17.1%) from the starting set of 8,127 will be finished in future work. Only 267 clones (3.2%) were aborted, which supports the success of our strategy for FLcDNA clone selection.

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BMC Genomics

ISSN: 1471-2164

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