Figure 2
Validation of sequence quality of FLcDNAs. Sequence accuracy was measured as the percentage of the 6,464 FLcDNAs which, with 100%, 95.0–99.9%, 90.0–94.9% or <90.0% of their sequence length, exceeded Phred30, Phred40, Phred50 or Phred60 sequence quality thresholds. All 6,464 FLcDNAs exceeded the Phred30 quality thresholds (less than 1 error in 103 sequenced nucleotides) over 100% of their sequence length. Even at the threshold level of Phred60 (less than 1 error in 106 sequenced nucleotides) the majority (74.1%) of the FLcDNA sequences met this very high sequence quality score over > 95.0% of their length.